Difference between revisions of "Part:BBa K3187023"

Revision as of 08:12, 19 October 2019

Tobacco Etch Virus (TEV) Protease Cleavage Site

Profile

Usage and Biology

The TEV potease cleavage site is the recognition sequence for a highly specific protease originated from the tobacco etch virus. The TEV protease recognizes the amino acid sequence Glu-Asn-Leu-Tyr-Phe-Gln-(Gly/Ser) and cleaves between Gln and (Gly/Ser) ^{[1] [2]} . We are using this recognition site to cut our protein in front of its polyG tag so it has a C-terminal polyG tag that can be used in a Sortase A reaction ^{[3] [4] [5]} .

References

1. ↑ J C Carrington and W G Dougherty, A viral cleavage site cassette: identification of amino acid sequences required for tobacco etch virus polyprotein processing. Proc Natl Acad Sci U S A. 1988 May; 85(10): 3391–3395. [1]
2. ↑ Product description TEV Protease, New England Biolabs [2]
3. ↑ Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering [3]
4. ↑ Proft, T. (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation [4]
5. ↑ Mao, H., Hart, S. A., Schink, A., and Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering [5]

Sequence and Features