Difference between revisions of "Part:BBa K2993003"

Revision as of 13:09, 17 October 2019

Fusion protein of ARRAY2-GSAT-C-TEV-6xHisTag

This part contains the C-TEV split protein fused to zincfinger array 2 via a GSAT-linker.

This fusion protein should be used with its counterpart which contains the C-TEV protein fused to another zinc-finger array with a binding affinity for a different DNA sequence. On our iGEMteam_Rotterdam2019 webpage, you can read all about how to use this part properly.

This part is special in its own way because it actually is a fusion of one half of a protein to a whole array of zinc-finger proteins. Much like a multi fusion protein.

The full sequence of this part is (we tried fitting it in the page width):

GAATTCGCGGCCGCTTCTAGAGGTTGACGGCTAGCTCAGTCCTAGGTACAG TGCTAGCTtcaagaGAAAGAGGGGACAAaCTAGATGGCCGGCCTGGAACCAGGCGAAAAA CCGTACAAATGCCCGGAATGTGGTAAAAGCTTCAGCCAGAGCGGCCATCTG ACCGAACACCAGCGTACGCATACCGGCGAAAAACCATATAAATGTCCGGAATGTGGCAAA TCTTTTAGCCGTGAAGATAACCTTCACACCCATCAGCGCACCCATACTGGTG AAAAACCGTATAAGTGCCCGGAATGTGGCAAAAGTTTTAGCAGCAAGAAACATCTGGCG GAACATCAACGCACCCACACCGGCGAAAAGCCGTATAAATGCCCGGAATGCG GCAAAAGCTTTAGCCGCACCGATACCCTGCGCGATCACCAACGCACTCACACAGGTGA GAAACCCTACAAGTGCCCAGAATGTGGAAAATCATTTAGCCGCGAGGATAAT TTACACACCCATCAACGTACGCATACAGGCGAAAAGCCATACAAGTGTCCCGAGTGTGG CAAAAGTTTCTCGCGCGAAGATAATTTGCATACGCACCAACGCACGCATACT GGCAAGAAGACAAGTACCGGCGGAGGGTCGGCAGGAGGGAGTGGTTCCGGATCTAGTG GTGGAAGCTCAGGAGCTAGTGGTACAGGCACTGCGGGTGGCACTGGCAGCG GTTCCGGTACTGGCTCTGGCACCGGCaagagcatgtctagcatggtgtcagacactagctgcacatt cccttcatctgatggcatattctggaagcattggattcaaaccaaggatgggcagtgtggcagtccat tagtatcaactagagatgggttcattgttggtatacactcagcatcgaatttcaccaacacaaacaattatttcacaa gcgtgccgaaaaacttcatggaattgttgacaaatcaggaggcgcagcagtgggttagtggttgg cgattaaatgctgactcagtattgtgggggggccataaagttttcatggtgaaacctgaagagccttttcagccagtt aaggaagcgactcaactcatgaatACCGGCCACCACCATCACCATCACCATCACTACTAGTAGCGGCCGCTGCAG

Sequence and Features