Difference between revisions of "Part:BBa K2984051"
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K2984051 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K2984051 SequenceAndFeatures</partinfo>
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==Characterization==
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<html>
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<figure>
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<img src="https://2019.igem.org/wiki/images/a/a2/T--Humboldt_Berlin--Konstruk_17.jpg" alt="colonies_total" width="500">
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<figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
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</figure>
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</html>
  
  

Revision as of 11:07, 17 October 2019


L1c-PsaD-bleRscp-YFP-RbcS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a bleomycin resistance with self cleaving peptide scp, a B2-B2 linker, a YFP mVenus marker, and the Rbcs2 terminator. This Level 1 construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1833
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 1833
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 4
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1833
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1833
    Illegal NgoMIV site found at 1453
    Illegal NgoMIV site found at 2310
  • 1000
    COMPATIBLE WITH RFC[1000]


Characterization

colonies_total
Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii