Difference between revisions of "Part:BBa K2984051"
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This Level 1 construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments. | This Level 1 construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments. | ||
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Revision as of 11:06, 17 October 2019
L1c-PsaD-bleRscp-YFP-RbcS2
This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a bleomycin resistance with self cleaving peptide scp, a B2-B2 linker, a YFP mVenus marker, and the Rbcs2 terminator. This Level 1 construct is designed for comparison of locus dependent expression through fluorescence intensity measuerments.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 1833
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 1833
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 4
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 1833
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 1833
Illegal NgoMIV site found at 1453
Illegal NgoMIV site found at 2310 - 1000COMPATIBLE WITH RFC[1000]