Difference between revisions of "Part:BBa K678004"
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Summary:in this part, we made contributions to the quantitative characterization data of mammalian PGK Promoter. We measured the strength of this promoter by expression change of firefly luciferase at continous time set after transfection of 6, 18, 30, 42 and 54 hours in HepG2 cell line, and made a curve showing how the strength of PGK promoter changes within 48 hours. | Summary:in this part, we made contributions to the quantitative characterization data of mammalian PGK Promoter. We measured the strength of this promoter by expression change of firefly luciferase at continous time set after transfection of 6, 18, 30, 42 and 54 hours in HepG2 cell line, and made a curve showing how the strength of PGK promoter changes within 48 hours. | ||
− | + | [[File:T--NUDT CHINA--result of PGK.png|130px|]] | |
Revision as of 05:00, 21 October 2019
PGK, mammalian promoter
Promoter for expression of genes in mammalian cells
Contribution: NUDT_CHINA 2019
Summary:in this part, we made contributions to the quantitative characterization data of mammalian PGK Promoter. We measured the strength of this promoter by expression change of firefly luciferase at continous time set after transfection of 6, 18, 30, 42 and 54 hours in HepG2 cell line, and made a curve showing how the strength of PGK promoter changes within 48 hours.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 143
Illegal PstI site found at 400 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 143
Illegal PstI site found at 400 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 143
Illegal PstI site found at 400 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 143
Illegal PstI site found at 400 - 1000COMPATIBLE WITH RFC[1000]