Difference between revisions of "Part:BBa K2984039"

Latest revision as of 22:34, 19 October 2019

L1c-PsaD-PETase-3xHA-RBCS2

This vector is a part of the Chlamy-HUB-Collection. This part is composed of the PsaD promoter, a B2-B2 linker, the PETase enzyme, a HA tag, and the Rbcs2 terminator. The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1705
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1244
Illegal PstI site found at 1705
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 968
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1705
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1705
1000
COMPATIBLE WITH RFC[1000]

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-This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">HA tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
+This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. This part is composed of the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2 linker</a>, the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984049">PETase enzyme</a>, a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984015">HA tag</a>, and the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The part can be used to express the PETase enzyme in <i>C. reinhardtii</i>. The 3xHA tag can be used to purify and detect the PETase enzyme.
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