Difference between revisions of "Part:BBa K2984035"

Revision as of 09:58, 17 October 2019

L1c-Psad-PETase-YFP-His-RbcS2

This vector is a part of the Chlamy-HUB-Collection. L1c plasmid containing PETase enzyme, fused to mVenus fluorescent marker for fluorecence meausrements of PETase concentration and His-tag for purification. The parts are flanked by the PsaD promoter and the Rbcs2 terminator. The part has a B2-B2 linker connecting the PsaD promoter and the PEtase. The construct is desgined to measure the expression level of PETase.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1705
Illegal PstI site found at 1919
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1244
Illegal PstI site found at 1705
Illegal PstI site found at 1919
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 968
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1705
Illegal PstI site found at 1919
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1705
Illegal PstI site found at 1919
Illegal NgoMIV site found at 2396
1000
COMPATIBLE WITH RFC[1000]

Characterization

Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii

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-<img src="https://2019.igem.org/File:T--Humboldt_Berlin--Konstrukt_8.jpeg" alt="colonies_total" width="500">
+<img src="https://2019.igem.org/wiki/images/9/95/T--Humboldt_Berlin--Konstrukt_8.jpeg" width="500">
 <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption>
 </figure>