Difference between revisions of "Part:BBa K2984033"

Revision as of 22:30, 19 October 2019

L1c-AR-ARS-YFP-Rbcs2

This vector is a part of the Chlamy-HUB-Collection. L1c plasmid containg arylsulfatase secretion signal ARS and mVenus fluorescent marker which are flanked by AR promoter and Rbcs2 terminator. The construct is designed to test for the efficency of the secretion signal and the expression rates of the AR Promoter in C.reinhardtii with fluorescence intensity measurements.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 953
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 268
Illegal PstI site found at 953
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 953
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 953
Illegal NgoMIV site found at 1430
1000
COMPATIBLE WITH RFC[1000]

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 This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. L1c plasmid containg arylsulfatase secretion signal <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984000">ARS</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984017">mVenus</a> fluorescent marker which are flanked by <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984009">AR promoter</a> and <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>.
-The construct is designed to test for the efficency of the secretion signal and the expression rates of the AR Promoter in C.reinhardtii with fluorescence intensity measurements.
+The construct is designed to test for the efficency of the secretion signal and the expression rates of the AR Promoter in <i>C.reinhardtii</i> with fluorescence intensity measurements.
 </html>