Difference between revisions of "Part:BBa K2912015"

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This part is the coding sequence (CDS) of a cytoplasmic protein TupA (GenBank: BAB07375.1), which catalyzes the conversion of glucuronic acid and L-glutamic acid to polyglucuronic acid and poly-γ-L-glutamic acid.  
 
This part is the coding sequence (CDS) of a cytoplasmic protein TupA (GenBank: BAB07375.1), which catalyzes the conversion of glucuronic acid and L-glutamic acid to polyglucuronic acid and poly-γ-L-glutamic acid.  
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K2912015 SequenceAndFeatures</partinfo>
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===Usage and Biology===
 
===Usage and Biology===
 
The lysis gene from colicin-producing strains of bacteria encodes the lysis protein which can cause the host cell to lyse as long as the protein is activated. On the basic of its biological function, we can use lysis gene to break our engineering E.coli and release the  interior cell contents when it's time to extract our product-shRNA by adding a Tryptophan attenuator between a RBS and lysis gene.
 
The lysis gene from colicin-producing strains of bacteria encodes the lysis protein which can cause the host cell to lyse as long as the protein is activated. On the basic of its biological function, we can use lysis gene to break our engineering E.coli and release the  interior cell contents when it's time to extract our product-shRNA by adding a Tryptophan attenuator between a RBS and lysis gene.

Revision as of 09:25, 17 October 2019

Trp_Lysis gene

This part is the coding sequence (CDS) of a cytoplasmic protein TupA (GenBank: BAB07375.1), which catalyzes the conversion of glucuronic acid and L-glutamic acid to polyglucuronic acid and poly-γ-L-glutamic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]