Difference between revisions of "Part:BBa K3226019:Design"
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===Design Notes=== | ===Design Notes=== | ||
| − | + | When PCR was performed, we were careful to use primers that insert a 6 × his tag between the start codon of RecA and the subsequent sequence. | |
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===Source=== | ===Source=== | ||
Revision as of 15:14, 17 October 2019
RecA+his
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 242
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 916
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
When PCR was performed, we were careful to use primers that insert a 6 × his tag between the start codon of RecA and the subsequent sequence.
Source
Deinococcus Radioduranse