Difference between revisions of "Part:BBa K3247007"

 
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<span class='h3bb'>Sequence and Features</span>
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===<span class='h3bb'>Sequence and Features</span>===
 
<partinfo>BBa_K3247007 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K3247007 SequenceAndFeatures</partinfo>
  

Revision as of 01:44, 22 October 2019


Para-B0034-iaaMH-L3S1P00

This cassette contains a Pcon-araC promoter, an Elowitz RBS (BBa_B0034), the genes iaaM and iaaH which together produce IAA, and a terminator. IAA (indole-3-acetic acid) or more commonly known as auxin is a plant hormone that increases root surface area and length and stimulates plant growth. There are many biosynthesis pathways to produce IAA from tryptophan, but this circuit uses two enzymes: iaaM and iaaH. The enzyme iaaM (tryptophan-2-monooxygenase) converts tryptophan into IAM (indole-3-acetamide) and the enzyme iaaH (indoleacetamide hydrolase) converts IAM into IAA (indole-3-acetic acid).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 507
    Illegal BamHI site found at 26
    Illegal BamHI site found at 1452
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 214
    Illegal NgoMIV site found at 2803
  • 1000
    COMPATIBLE WITH RFC[1000]