Difference between revisions of "Part:BBa K3202058"
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<partinfo>BBa_K3202058 short</partinfo> | <partinfo>BBa_K3202058 short</partinfo> | ||
− | + | Alternative version of BBa_K3202048, sfGFP is replaced by recombinase TP901 | |
+ | |||
+ | In former studies, we find out that AraC-Pc-pBAD-LacI system can provide the most rigorous results. Therefore, we created the first bistable system in our design by repLacIng sfGFP with recombinases. | ||
Latest revision as of 06:43, 19 October 2019
AraC-Pc-pBAD-LacI-B0034-TP901-T500-T1/TE-MicCsRNA-sRNA Binding site-PA1/O4
Alternative version of BBa_K3202048, sfGFP is replaced by recombinase TP901
In former studies, we find out that AraC-Pc-pBAD-LacI system can provide the most rigorous results. Therefore, we created the first bistable system in our design by repLacIng sfGFP with recombinases.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 239
Illegal BamHI site found at 532 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 74
Illegal AgeI site found at 367 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 56
Illegal SapI site found at 349