Difference between revisions of "Part:BBa K103004"
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<partinfo>BBa_K103004 short</partinfo> | <partinfo>BBa_K103004 short</partinfo> | ||
| − | Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003). Codon | + | Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003). |
| + | *Z<sub>SPA-1</sub>-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris) | ||
| + | *We have used Z<sub>SPA-1</sub> protein fusions in our 'hunter and prey' selection system | ||
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| − | <!-- Uncomment this to enable Functional Parameter display | + | <!-- Uncomment this to enable Functional Parameter display |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K103004 parameters</partinfo> | <partinfo>BBa_K103004 parameters</partinfo> | ||
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Revision as of 19:42, 21 October 2008
protein ZSPA-1
Artificially created strong interactor of B domain of Staphylococcal protein A (BBa_K103003). Codon usage optimized for expresion in E.coli. It autoaggregates easily even in fusion with soluble protein. Preferentially binds B domain of Staphylococcal protein A (BBa_K103003).
- ZSPA-1-tagged proteins can be easily purified (more 99% of the protein is present in postsonication debris)
- We have used ZSPA-1 protein fusions in our 'hunter and prey' selection system
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]