Difference between revisions of "Part:BBa K2960011:Design"
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===References=== | ===References=== | ||
| + | Wang, J., Wang, C., Li, J., Bai, P., Li, Q., Shen, M., … Zhao, J. (2018). Comparative Genomics of Degradative Novosphingobium Strains With Special Reference to Microcystin-Degrading Novosphingobium sp. THN1. Frontiers in Microbiology, 9. doi: 10.3389/fmicb.2018.02238 | ||
Latest revision as of 01:17, 21 October 2019
The mlrF enzyme
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The mlrF gene sequence has been codon-optimized for expression in E. coli.
The construct was designed to be RFC[10] compatible.
Source
Sphingopyxis sp. genomic sequence. Jin H, Nishizawa T, Guo Y, Nishizawa A, Park H-D, Kato H, Tsuji K, Harada K-I. 2018. Complete genome sequence of a microcystin-degrading bacterium, Sphingosinicella microcystinivorans strain B-9. Microbiol Resour Announc 7:e00898-18. https://doi.org/10.1128/MRA.00898-18
References
Wang, J., Wang, C., Li, J., Bai, P., Li, Q., Shen, M., … Zhao, J. (2018). Comparative Genomics of Degradative Novosphingobium Strains With Special Reference to Microcystin-Degrading Novosphingobium sp. THN1. Frontiers in Microbiology, 9. doi: 10.3389/fmicb.2018.02238