Difference between revisions of "Part:BBa K3292001:Design"
Line 14: | Line 14: | ||
Promoter Bba_K2406020 | Promoter Bba_K2406020 | ||
− | RBS | + | RBS Bba_J34801 |
Terminator Bba_0010 | Terminator Bba_0010 | ||
sfGFP BBa_I746916 | sfGFP BBa_I746916 |
Revision as of 01:13, 17 October 2019
T7-LacO Promoter + strong RBS + sialidase + sfGFP + 6x his-tag + double terminator
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 721
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1986
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 646
Illegal BsaI.rc site found at 510
Illegal BsaI.rc site found at 1647
Illegal SapI.rc site found at 2055
Design Notes
The purification of the whole construct presented some disadvantages when using imidazole.
Source
Promoter Bba_K2406020 RBS Bba_J34801 Terminator Bba_0010 sfGFP BBa_I746916 The sialidase coding sequence was retrieved from UNIPROT (Q02834 (NANH_MICVI))