Difference between revisions of "Part:BBa K138000"

 
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<partinfo>BBa_K138000 short</partinfo>
 
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Revision as of 21:14, 4 November 2008

NifHp in BioBrick Compatible Leucine Landing Pad

The NifH promoter was subcloned into the leucine landing pad using Xba and Nde. Other genetic elements can be subcloned behind the promoter using either NdeI/SpeI or NdeI/PstI (or other combinations). The linker region is the following: EcorI-XbaI- NifHp- NdeI- PacI-SpeI-PstI.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]