Difference between revisions of "Promoters/Catalog"
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The registry has a large collection of [[Promoters]] available.  We categorize promoters using two different parameters.  First, most promoters are designed for a particular RNA polymerase holoenzyme, e.g ''E. coli'' RNA polymerase with σ70, or T7 RNA polymerase.  Second, promoters may be either unregulated (constitutive), positively regulated (inducible) or negatively regulated (repressible).  So for example, if you want express a gene in growing ''E. coli'' at a with little expression before hand, you might choose a lacI repressible σ70 promoter.  
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The registry has a large collection of [[Promoters]] available.  We categorize promoters using two different parameters.  First, most promoters are designed for a particular RNA polymerase (RNAP) holoenzyme, e.g ''E. coli'' RNAP with a particular sigma factor (for example σ70) that confers specificity on the RNAP holoenzyme , or the RNAP from the T7 bacteriophage.  Second, promoters are categorized by the manner in which they are regulated.  For example a promoter may be unregulated (constitutive), positively regulated (inducible) or negatively regulated (repressible).  Regulated promoters allow you to "program" biological systems.  For example, you can perform logic (e.g. "If protein X is present, promoter Y is off") or ensure that a particular gene is only expressed when a particular small molecule is present in the media.
  
 
The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.
 
The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.
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{|align="center" border="1" cellpadding="0" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;" width="100%"
 
{|align="center" border="1" cellpadding="0" cellspacing="0" style="border:#c9c9c9 1px solid; margin: 1em 1em 1em 0; border-collapse: collapse;" width="100%"
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!colspan="2" rowspan="3"|Regulation
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!rowspan="3" width="120px"|Regulation
 
!colspan="3"|RNA Polymerase
 
!colspan="3"|RNA Polymerase
 
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|-
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|
 
|
 
|-style="height:58px"  
 
|-style="height:58px"  
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|rowspan="4" width="50pt" align="center" |[[Image:BCInducibleIcon.png]][[Image:ConstitutiveIcon.png]][[Image:BCRepressibleIcon.png]][[Image:BCMultipleIcon.png]]
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|rowspan="4" width="50pt" align="center" |
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|width="50pt" |Inducible
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{|cellpadding="0" cellspacing="0"
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| valign="center" style="height:58px" align="center" width="80px"|Positive
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|width="20px"|[[Image:BCInducibleIcon.png]]
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|-style="height:58px" align="center" valign="center"
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|Constitutive
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|[[Image:ConstitutiveIcon.png]]
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|-style="height:58px" align="center" valign="center"
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|Negative
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|[[Image:BCRepressibleIcon.png]]
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|-style="height:58px" align="center" valign="center"
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|Multiple
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|[[Image:BCMultipleIcon.png]]
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|}
 
|align="center"| See <count_promoters inducible sigma70></count_promoters> promoters
 
|align="center"| See <count_promoters inducible sigma70></count_promoters> promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|-style="height:58px"  
 
|-style="height:58px"  
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|Constitutive
 
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|-style="height:58px"  
 
|-style="height:58px"  
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|Repressible
 
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|-style="height:38px"  
 
|-style="height:38px"  
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|Multiple
 
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters
 
|align="center"| See X promoters

Revision as of 16:06, 28 October 2008

The registry has a large collection of Promoters available. We categorize promoters using two different parameters. First, most promoters are designed for a particular RNA polymerase (RNAP) holoenzyme, e.g E. coli RNAP with a particular sigma factor (for example σ70) that confers specificity on the RNAP holoenzyme , or the RNAP from the T7 bacteriophage. Second, promoters are categorized by the manner in which they are regulated. For example a promoter may be unregulated (constitutive), positively regulated (inducible) or negatively regulated (repressible). Regulated promoters allow you to "program" biological systems. For example, you can perform logic (e.g. "If protein X is present, promoter Y is off") or ensure that a particular gene is only expressed when a particular small molecule is present in the media.

The value of the parts in this catalog increases the more you use them and report your results so please remember to contribute back any data (positive or negative!) that you obtain for any promoters in the catalog.


Regulation RNA Polymerase
E. coli T7
σ70 σS
Positive BCInducibleIcon.png
Constitutive ConstitutiveIcon.png
Negative BCRepressibleIcon.png
Multiple BCMultipleIcon.png
See Unspecified21 promoters See X promoters See X promoters
See X promoters See X promoters See X promoters
See X promoters See X promoters See X promoters
See X promoters See X promoters See X promoters