Difference between revisions of "Part:BBa K3183202"
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<partinfo>BBa_K3183202 short</partinfo> | <partinfo>BBa_K3183202 short</partinfo> | ||
| − | AgrBD is used by C. difficile to produce its quorum signalling molecule, also known as the auto-inducer peptide (AIP). This part is designed to be used in E. coli to enable purification and characterisation of thie elusive protein, which is linked to toxin synthesis in C. difficile | + | AgrBD is used by C. difficile to produce its quorum signalling molecule, also known as the auto-inducer peptide (AIP). This part is designed to be used in E. coli to enable purification and characterisation of thie elusive protein, which is linked to toxin synthesis in <i>C. difficile</i> <sup>1</sup>. This part also contains a His tag after each protein to allow individual purification. |
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<partinfo>BBa_K3183202 parameters</partinfo> | <partinfo>BBa_K3183202 parameters</partinfo> | ||
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| + | ===Reference=== | ||
| + | 1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14. | ||
Latest revision as of 03:15, 22 October 2019
pET28A-AgrBD
AgrBD is used by C. difficile to produce its quorum signalling molecule, also known as the auto-inducer peptide (AIP). This part is designed to be used in E. coli to enable purification and characterisation of thie elusive protein, which is linked to toxin synthesis in C. difficile 1. This part also contains a His tag after each protein to allow individual purification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Reference
1. Darkoh, Charles, et al. “Toxin Synthesis by Clostridium Difficile Is Regulated through Quorum Signaling.” MBio, vol. 6, no. 2, May 2015, pp. e02569-14. mbio.asm.org, doi:10.1128/mBio.02569-14.