Difference between revisions of "Part:BBa K3165013:Design"

 
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===Design Notes===
 
===Design Notes===
−
Single base-pair mutation at the 48th position and codon optimised for E. coli
+
Single base-pair mutation at the 48th position converting the start codon (ATG) to ATC encoding Isoleucine and codon optimised for <i>Escherichia coli</i>.
  
  

Revision as of 07:20, 19 October 2019


i^2mCherry (Ile)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 679
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Single base-pair mutation at the 48th position converting the start codon (ATG) to ATC encoding Isoleucine and codon optimised for Escherichia coli.


Source

The part is derived from the existing mCherry sequence in the iGEM registry (BBa_J18932).

References