Difference between revisions of "Part:BBa K3051005"
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<partinfo>BBa_K3051005 short</partinfo>
 
<partinfo>BBa_K3051005 short</partinfo>
  
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This is a lipase from Bacillus Subtilis XF-1 it was found in during a metagenomic analysis of restaurant wastewater containing high levels of FOG (fat oil and grease). It has a kDa of 24.1.
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This is a lipase from Bacillus Subtilis XF-1 it was found during a metagenomic analysis of restaurant wastewater containing high levels of FOG (fat oil and grease); we therefore believe that it . It has a kDa of 24.1.
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It was tested and characterized using a p nitrophenol octanoate assay. Details of this assay are available on the iGem 2019 wiki page.  
 
It was tested and characterized using a p nitrophenol octanoate assay. Details of this assay are available on the iGem 2019 wiki page.  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 20:00, 16 October 2019


Bacillus Subtilis Lipase

This is a lipase from Bacillus Subtilis XF-1 it was found during a metagenomic analysis of restaurant wastewater containing high levels of FOG (fat oil and grease); we therefore believe that it . It has a kDa of 24.1.

It was tested and characterized using a p nitrophenol octanoate assay. Details of this assay are available on the iGem 2019 wiki page.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

The part contains a EstA triacylglycerol lipase conserved region, which makes up a large proportion of its total length.