Difference between revisions of "Part:BBa K3051005"

Revision as of 19:58, 16 October 2019

Bacillus Subtilis Lipase

This is a lipase from Bacillus Subtilis XF-1 it was found in during a metagenomic analysis of restaurant wastewater containing high levels of FOG (fat oil and grease). It has a kDa of 24.1.

It was tested and characterized using a p nitrophenol octanoate assay. Details of this assay are available on the iGem 2019 wiki page.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

The part contains a EstA triacylglycerol lipase conserved region, which makes up a large proportion of its total length.

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K3051005 short</partinfo>
-This is a lipase from Bacillus Subtilis XF-1 it was found in during a metagenomic analysis of restaurant wastewater containing high levels of FOG (fat oil and grease). It was tested and characterized using a p nitrophenol octanoate assay. Details of this assay are available on the iGem 2019 wiki page.
+This is a lipase from Bacillus Subtilis XF-1 it was found in during a metagenomic analysis of restaurant wastewater containing high levels of FOG (fat oil and grease). It has a kDa of 24.1.
+It was tested and characterized using a p nitrophenol octanoate assay. Details of this assay are available on the iGem 2019 wiki page.
 <!-- Add more about the biology of this part here
@@ Line 12: / Line 14: @@
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K3051005 SequenceAndFeatures</partinfo>
+The part contains a EstA triacylglycerol lipase conserved region, which makes up a large proportion of its total length.
 <!-- Uncomment this to enable Functional Parameter display