Difference between revisions of "Part:BBa K2984048"

Revision as of 20:07, 16 October 2019

L1c-PsaD-bleRscp-ARS-PETase-SP20-RbcS2

This part is composed of the PsaD promoter (BBa_K2984008), bleomycin resistance with self cleaving peptide scp (BBa_K2984045), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), the secretion enhancing SP20 glycomodule (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018).

The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every bleomycin moleucle, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:1 to the antibiotic. By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 2249
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 1973
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 1607
1000
COMPATIBLE WITH RFC[1000]

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 <partinfo>BBa_K2984048 short</partinfo>
-This part is composed of the PsaD promoter (BBa_K2984008), bleomycin resistance with self cleaving peptide scp (BBa_K2984045), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), the secretion enhancing SP20 glycomodule (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every bleomycin resistance, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:1 to the antibiotic. By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme.
+This part is composed of the PsaD promoter (BBa_K2984008), bleomycin resistance with self cleaving peptide scp (BBa_K2984045), the ARS secretion signal (BBa_K2984000), the PETase enzyme (), the secretion enhancing SP20 glycomodule (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018).
+The part can be used to express and secrete the PETase enzyme in C. reinhardtii. The bleomycin resistance leads to a higher expression of the enzyme, due to its working mechanism. For every bleomycin moleucle, the bleomycin resistance gene must be expressed once. The resistance works in a ratio of 1:1 to the antibiotic. By having the resistance in the same open reading frame, the enzyme is also expressed, leading to a higher expression of the enzyme.
 <!-- Add more about the biology of this part here