Difference between revisions of "Part:BBa K3117006"

Line 3: Line 3:
 
<partinfo>BBa_K3117006 short</partinfo>
 
<partinfo>BBa_K3117006 short</partinfo>
  
The part BBa_K3117006 is a secretory leader sequence for eucaryotic expression. With the help of Igk, protein can be directly secreted and harvested out of the supernatant.
+
The part BBa_K3117006 is a secretory leader sequence for eucaryotic expression. With the help of Igk, proteins can be directly secreted and harvested out of the supernatant. Igk is normally fused to the N-terminus of the protein of interest.
  
  
Line 9: Line 9:
 
===Usage and Biology===
 
===Usage and Biology===
  
 +
The Team 2019 FAU_Erlangen used the Igk leader sequence to secrete their proteins (bispecific antibodies).
 
<!-- -->
 
<!-- -->
 
<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 13:28, 18 October 2019


Igk leader sequence

The part BBa_K3117006 is a secretory leader sequence for eucaryotic expression. With the help of Igk, proteins can be directly secreted and harvested out of the supernatant. Igk is normally fused to the N-terminus of the protein of interest.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]