Difference between revisions of "Part:BBa K3002027"
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This basic part contains the PAR promoter and a 5'UTR (A1-B2) for Chlamydomonas reinhardtii and was built as a part of the Kaiser Collection. Combined with a CDS, and a terminator, this level 0 construct leads to a high expression of a target protein. Especially in combination with the part <a href="https://parts.igem.org/Part:BBa_K3002000">BBa_K3002000</a>(Spectinomycin resistance gene aadA) a high expression can be observed. As this part is a combination of the promoters HSP70A (upstream) and RBCS2, no heat shock is needed as a transcription activator (<a href="https://doi.org/10.1046/j.1365-313x.2000.00652.x"> Schroda, M.,2000</a>). | This basic part contains the PAR promoter and a 5'UTR (A1-B2) for Chlamydomonas reinhardtii and was built as a part of the Kaiser Collection. Combined with a CDS, and a terminator, this level 0 construct leads to a high expression of a target protein. Especially in combination with the part <a href="https://parts.igem.org/Part:BBa_K3002000">BBa_K3002000</a>(Spectinomycin resistance gene aadA) a high expression can be observed. As this part is a combination of the promoters HSP70A (upstream) and RBCS2, no heat shock is needed as a transcription activator (<a href="https://doi.org/10.1046/j.1365-313x.2000.00652.x"> Schroda, M.,2000</a>). | ||
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Revision as of 18:53, 16 October 2019
pAR promoter for Chlamydomonas reinhardtii (Phytobrick) This basic part contains the PAR promoter and a 5'UTR (A1-B2) for Chlamydomonas reinhardtii and was built as a part of the Kaiser Collection. Combined with a CDS, and a terminator, this level 0 construct leads to a high expression of a target protein. Especially in combination with the part BBa_K3002000(Spectinomycin resistance gene aadA) a high expression can be observed. As this part is a combination of the promoters HSP70A (upstream) and RBCS2, no heat shock is needed as a transcription activator ( Schroda, M.,2000).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 249
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]