Difference between revisions of "Part:BBa K3002027"

 
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<partinfo>BBa_K3002027 short</partinfo>
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This basic part contains the PAR promoter and a 5'UTR (A1-B2) for Chlamydomonas reinhardtii and was built as a part of the Kaiser Collection. Combined with a CDS, and a terminator, this level 0 construct leads to a high expression of a target protein. Especially in combination with the part <a href="https://parts.igem.org/Part:BBa_K3002000">BBa_K3002000</a>(Spectinomycin resistance gene aadA) a high expression can be observed. As this part is a combination of the promoters HSP70A (upstream) and RBCS2, no heat shock is needed as a transcription activator (<a href="https://doi.org/10.1046/j.1365-313x.2000.00652.x"> Schroda, M.,2000</a>).
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</html>
  
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<!-- Add more about the biology of this part here
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K3002027 SequenceAndFeatures</partinfo>
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===Functional Parameters===
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<partinfo>BBa_K3002027 parameters</partinfo>
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Revision as of 18:52, 16 October 2019

pAR promoter for Chlamydomonas reinhardtii (Phytobrick) >html> This basic part contains the PAR promoter and a 5'UTR (A1-B2) for Chlamydomonas reinhardtii and was built as a part of the Kaiser Collection. Combined with a CDS, and a terminator, this level 0 construct leads to a high expression of a target protein. Especially in combination with the part <a href="https://parts.igem.org/Part:BBa_K3002000">BBa_K3002000</a>(Spectinomycin resistance gene aadA) a high expression can be observed. As this part is a combination of the promoters HSP70A (upstream) and RBCS2, no heat shock is needed as a transcription activator (<a href="https://doi.org/10.1046/j.1365-313x.2000.00652.x"> Schroda, M.,2000</a>). </html>

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]