Difference between revisions of "Part:BBa K3081053"

Revision as of 00:03, 21 October 2019

AHL receiver device to expression of dCas9 and sgRNA

Quorum sensing system is widely used in gene circuit design as a sensor of cell density. As a transcription factor, LuxR senses AHL and then activate the transcript of plux. In our project, we want dCas9 to express when bacteria density is high. So we put the dCas9 gene under the control of plux, and get this composite part. And we can use golden-gate assembly methods to change the sequence of sgRNA.

Figure1 We transfer this part in E. coli ,BL21(DE3), and culture them in M9 medium containing a series of AHL concentration for 6 hours. Receiver cells show remarkable dose-dependent change in length with addition of AHL.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 1321
Illegal XhoI site found at 5538
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 5728
Illegal BsaI.rc site found at 1004
Illegal BsaI.rc site found at 5324

@@ Line 4: / Line 4: @@
 Quorum sensing system is widely used in gene circuit design as a sensor of cell density. As a transcription factor, LuxR senses AHL and then activate the transcript of plux. In our project, we want dCas9 to express when bacteria density is high. So we put the dCas9 gene under the control of plux, and get this composite part. And we can use golden-gate assembly methods to change the sequence of sgRNA.
+https://2019.igem.org/wiki/images/7/72/T--Peking--QS3.png
+Figure1 We transfer this part in <i>E. coli</i> ,BL21(DE3), and culture them in M9 medium containing a series of AHL concentration for 6 hours. Receiver cells show remarkable dose-dependent change in length with addition of AHL.
 <!-- Add more about the biology of this part here