Difference between revisions of "Part:BBa K2999010"

Revision as of 18:17, 16 October 2019

pPA2069+RBS+PrtN

PrtN, a lysozyme activating protein of Pseudomonas aeruginosa PAO1, was linked to PA2069, a promoter regulated by HSL signal molecule of PAO1 quorum sensing system, as a delayed expression system to cleave PAO1.

Usage and Biology

Figure 1. The Yau China team cultured the experimental strain and the control group in LB liquid medium respectively, and detected OD600 every 6 hours with enzyme labeling instrument. It can be seen that the experimental strain began to decline obviously after growing to the stable stage.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 365
Illegal XhoI site found at 698
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 96
Illegal NgoMIV site found at 271
Illegal NgoMIV site found at 297
Illegal NgoMIV site found at 482
Illegal NgoMIV site found at 602
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 989

@@ Line 5: / Line 5: @@
 PrtN, a lysozyme activating protein of Pseudomonas aeruginosa PAO1, was linked to PA2069, a promoter regulated by HSL signal molecule of PAO1 quorum sensing system, as a delayed expression system to cleave PAO1.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+[[File:T--YAU-China--iGEM2019-BBa -8.png|500px|thumb|none|Figure 1. The Yau China team cultured the experimental strain and the control group in LB liquid medium respectively, and detected OD600 every 6 hours with enzyme labeling instrument. It can be seen that the experimental strain began to decline obviously after growing to the stable stage.]]
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