Difference between revisions of "Part:BBa K3171175"

 
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===Usage and Biology===
 
===Usage and Biology===
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Improvisation of the pTET promoter to enhance its strength in E. coli. Using a synthetic promoter library on pTet (BBa_R0040) in order to enhance function and inducibility.
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In order to enhance function and inducibility. We optimized tetracycline-inducibility in E. coli by creating randomizations in the sequence to obtain arbitrary promoters. The screening was done based on quantifications of the fluorescence intensity measured. The best version of the promoter was determined based on fold change in the fluorescence intensity and low basal level. This promoter is also used in the composite part with mCherry fusion protein.
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Revision as of 20:24, 19 October 2019


pTet-mCherry optimized in E. coli

Enhanced pTET mCherry for E. coli

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]