Difference between revisions of "Part:BBa K3053001"
LokshananR (Talk | contribs) |
|||
| Line 3: | Line 3: | ||
<partinfo>BBa_K3053001 short</partinfo> | <partinfo>BBa_K3053001 short</partinfo> | ||
| − | This part consists of the RBS with the UTR that targets the beta lactamase mRNA and represses its expression | + | This part consists of the RBS with the UTR that targets the beta lactamase mRNA and represses its expression by forming a double stranded mRNA. |
| + | |||
| + | <!-- Add more about the biology of this part here--> | ||
| − | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
| + | <p>The antisense region is flanked by an RBS sequence on the 5' end and a complementary RBS sequence on the 3' end. The RBS forms a duplex by binding to its complementary RBS sequence in the absence of antibiotic resistance gene & thus, inactivates the expression of the Cro gene. When the antibiotic resistant gene is present, the RBS binds to the complement RBS in the mRNA of the resistant bacterium & enables the expression of the Cro gene.</p> | ||
<!-- --> | <!-- --> | ||
Latest revision as of 16:02, 21 October 2019
RBS + UTR of beta lactamase
This part consists of the RBS with the UTR that targets the beta lactamase mRNA and represses its expression by forming a double stranded mRNA.
Usage and Biology
The antisense region is flanked by an RBS sequence on the 5' end and a complementary RBS sequence on the 3' end. The RBS forms a duplex by binding to its complementary RBS sequence in the absence of antibiotic resistance gene & thus, inactivates the expression of the Cro gene. When the antibiotic resistant gene is present, the RBS binds to the complement RBS in the mRNA of the resistant bacterium & enables the expression of the Cro gene.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]