Difference between revisions of "Part:BBa K3081122"
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<partinfo>BBa_K3081122 short</partinfo> | <partinfo>BBa_K3081122 short</partinfo> | ||
| − | This part is an improvement of dCas9-linker-sfGFP(BBa_K3081119), in which we add a degradation signal peptide ssrA to dCas9. | + | This part is an improvement of dCas9-linker-sfGFP(<partinfo>BBa_K3081119</partinfo>), in which we add a degradation signal peptide ssrA to dCas9.In this part,a fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose. |
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Revision as of 15:58, 19 October 2019
dCas9-linker-sfGFP-ssrA
This part is an improvement of dCas9-linker-sfGFP(BBa_K3081119), in which we add a degradation signal peptide ssrA to dCas9.In this part,a fluorescent protein sfGFP is fused to the C-terminal of dCas9 for quantitative characterization. A degradation tag ssrA is added to the end of sfGFP to alleviate the burden caused by dCas9. The expression of this fusion protein is driven by pBAD promoter, which can be triggered by arabinose.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1099
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3378
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 4147