Difference between revisions of "Part:BBa K3286043"
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The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase. | The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase. | ||
| + | ===References=== | ||
| + | |||
| + | <ul><p>Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004</p> | ||
| + | <p>Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304. </p> | ||
| + | <p>Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110</p> | ||
| + | </ul> | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 15:58, 20 October 2019
Native bacteriophage Lambda PR and PRM promoter, OR operators and 5' UTR
The native bacteriophage Lambda PR and PRM promoter of bacteriophage Lambda, with the operator regions OR1, OR2 and OR3, and the PR 5'UTR.
The PRM promoter can be regulated by the native bacteriophage lambda proteins CI and CRO. The CI protein regulates the RPM promoter in a positive sense by creating a docking station for RNA polymerase and the CRO protein can regulated the PRM promoter in a negative sense by shielding the promoter region for binding of RNA polymerase.
References
Atsumi, S., & Little, J. W. (2004). Regulatory circuit design and evolution using phage λ. Genes and Development, 18(17), 2086–2094. https://doi.org/10.1101/gad.1226004
Court, D. L., Oppenheim, A. B., & Adhya, S. L. (2007). A new look at bacteriophage lambda genetic networks. Journal of Bacteriology, 189(2), 298–304.
Liu, X., Jiang, H., Gu, Z., & Roberts, J. W. (2013). High-resolution view of bacteriophage lambda gene expression by ribosome profiling. Proceedings of the National Academy of Sciences, 110(29), 11928–11933. https://doi.org/10.1073/pnas.1309739110
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]