Difference between revisions of "Part:BBa K3179102"

Revision as of 22:44, 19 October 2019

pCMV-rtTA3

Sequence and Features

Assembly Compatibility:

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 <!-- Add more about the biology of this part here
 ===Usage and Biology===
+This part consist with promoter pCMV can basic part rtTA3. pCMV is a strong mammalian expression promoter derived from human cytomegalovirus, usually used for routine expression, with high expression levels but may be inhibited in some cells. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.
+In our experiment, pCMV-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.<br style="clear: both" />
+THERE SHOULD BE A FIGURE.<br style="clear: both" />
+This result shows the influence of two kinds of promoter pCMV and hEF1α to the expression.
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 <span class='h3bb'>Sequence and Features</span>