Difference between revisions of "Part:BBa K3179103"
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pUbC-rtTA3 | pUbC-rtTA3 | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part consists of promoter pUbC and basic part rtA3. pUbC from human ubiquitin C gene and is a mammalian promoter. pUbC is usually used for regular expression and has a lower expression. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.<br style="clear: both" /> | ||
+ | In our experiment, pUbC-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3. | ||
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Latest revision as of 03:47, 20 October 2019
pUbC-rtTA3
pUbC-rtTA3
Usage and Biology
This part consists of promoter pUbC and basic part rtA3. pUbC from human ubiquitin C gene and is a mammalian promoter. pUbC is usually used for regular expression and has a lower expression. rtTA is a key component of Tet-On system, and rtTA3 is third generation rtTA. rtTA3 is an activation factor of pTRE. rtTA3 isn’t active when it is present alone, and it can be active when combine with doxycycline (DOX), then the complex can combine to pTRE and start the downstream transcription. When DOX get removed the rtTA3 will became no activation again.
In our experiment, pUbC-rtTA3 is used to test the effect of the influence of different promoter to the expression of rtTA3.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal XbaI site found at 999
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 203
- 23INCOMPATIBLE WITH RFC[23]Illegal XbaI site found at 999
- 25INCOMPATIBLE WITH RFC[25]Illegal XbaI site found at 999
Illegal NgoMIV site found at 473
Illegal AgeI site found at 209 - 1000COMPATIBLE WITH RFC[1000]