Difference between revisions of "Part:BBa K3081113"
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<partinfo>BBa_K3081113 short</partinfo> | <partinfo>BBa_K3081113 short</partinfo> | ||
| − | + | The 20 bp sgRNA targets to the duplex unwinding element (DUE) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. For more detailed information, see <partinfo>BBa_K3081058</partinfo> | |
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Revision as of 14:58, 19 October 2019
a 20bp sequence targeting the DNA unwinding element on E.coli oriC
The 20 bp sgRNA targets to the duplex unwinding element (DUE) on E.coli genome replication initiation region, OriC. Not considered to be DnaA binding box,blocking this site has a relatively mild arrest and inhibition of genome replication initiation. For more detailed information, see BBa_K3081058
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]