Difference between revisions of "Part:BBa K3040107"
 
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<partinfo>BBa_K3040107 short</partinfo>
 
<partinfo>BBa_K3040107 short</partinfo>
  
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Tes A is a heterologous thioesterase, which is able to hydrolyze fatty acyl-CoAs to free fatty acids. Here, we combine Tes A with pFadBA (wild type) together as a new promoter, Tes A pFadBA, to see if the sensitivity range of fatty acid concentration lowers due to the increase of endogenous fatty acid.
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In the microbial fatty acid biosynthesis pathway, carbon source such as glucose or fatty acid will be metabolized to Acyl-ACP. Nevertheless, the accumulation of Acyl-ACP will give negative feedback to the fatty acid biosynthesis. Thus, TesA gene which encodes a thioesterase will hydrolyze these Acyl-ACP and subsequently produce a significant level of free fatty acid.  
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here
 
===Usage and Biology===
 
===Usage and Biology===

Latest revision as of 09:30, 20 October 2019


TesA

In the microbial fatty acid biosynthesis pathway, carbon source such as glucose or fatty acid will be metabolized to Acyl-ACP. Nevertheless, the accumulation of Acyl-ACP will give negative feedback to the fatty acid biosynthesis. Thus, TesA gene which encodes a thioesterase will hydrolyze these Acyl-ACP and subsequently produce a significant level of free fatty acid. Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal suffix found in sequence at 981
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 982
    Illegal PstI site found at 996
    Illegal NotI site found at 989
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal suffix found in sequence at 982
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 982
    Illegal PstI site found at 996
  • 1000
    COMPATIBLE WITH RFC[1000]