Difference between revisions of "Part:BBa K3196033:Design"

Latest revision as of 07:09, 21 October 2019

FLO10-αpro-Geneart Laccase

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 349
Illegal BglII site found at 886
Illegal BglII site found at 1582
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Design Notes

This part is an improvement of part Baa_K500002, which codes the Geneart Laccase. Geneart Laccase is a polyphenol oxidase, which belongs to the family of blue multicopper oxidases. Laccases oxidize a broad range of substrates, preferably phenolic compounds. As we found in our project, the signal peptide has a strong influence on protein secretory. We have references show that the FLO10-αpro contributes a lot to the laccase secretory in yeast. As a part, we want to amplify the response of the promoter.

Source

BBa_K500002

References

Yiping Shi, Xue Zhou, Meirong Nu, Jianhui Lin, Yong Tao, Jianzhong Huang. Effect of signal peptides on the expression of laccase in Pichia pastoris. Acta Microbiologica Sinica, 54(12): 1446-1452.

Revision as of 15:36, 16 October 2019 (view source) Registry (Talk \| contribs)		Latest revision as of 07:09, 21 October 2019 (view source) GlacierHOLE (Talk \| contribs)
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	===References===		===References===
		+	Yiping Shi, Xue Zhou, Meirong Nu, Jianhui Lin, Yong Tao, Jianzhong Huang. Effect of signal peptides on the expression of laccase in Pichia pastoris. Acta Microbiologica Sinica, 54(12): 1446-1452.