Difference between revisions of "Part:BBa K2999008"

 
 
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<partinfo>BBa_K2999008 short</partinfo>
 
<partinfo>BBa_K2999008 short</partinfo>
  
We amplified T7 promoter from pET22b plasmid.
+
We amplified T7 promoter from pET22b plasmid.After that we added Nde I after the promoter
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 16:56, 16 October 2019


T7 promoter

We amplified T7 promoter from pET22b plasmid.After that we added Nde I after the promoter

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 60
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 60
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 60
  • 1000
    COMPATIBLE WITH RFC[1000]