Difference between revisions of "Part:BBa K3007029"
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It works as a uric acid reporter. Pc is a constitutive promoter, Pcp6 promoter, and it promotes the expression of HucR and YgfU. If the concentration of uric acid (UA) in environment is low, HucR will bind to PhucR, which inhibits the expression of downstream reporter, dsRed. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from PhucR, and induces the fluorescent protein expression. | It works as a uric acid reporter. Pc is a constitutive promoter, Pcp6 promoter, and it promotes the expression of HucR and YgfU. If the concentration of uric acid (UA) in environment is low, HucR will bind to PhucR, which inhibits the expression of downstream reporter, dsRed. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from PhucR, and induces the fluorescent protein expression. | ||
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| + | The part is equal to BBa_K3007004, because of a mistake in part document. | ||
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Revision as of 14:55, 16 October 2019
Expression device of dsRed under uric-acid-responsive regulatory system
It works as a uric acid reporter. Pc is a constitutive promoter, Pcp6 promoter, and it promotes the expression of HucR and YgfU. If the concentration of uric acid (UA) in environment is low, HucR will bind to PhucR, which inhibits the expression of downstream reporter, dsRed. When extracellular UA is present, YgfU can transport UA into the cytoplasm, which leads HucR dissociates from PhucR, and induces the fluorescent protein expression.
The part is equal to BBa_K3007004, because of a mistake in part document.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal XbaI site found at 3113
Illegal SpeI site found at 917
Illegal PstI site found at 140
Illegal PstI site found at 510
Illegal PstI site found at 3210 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal NheI site found at 1085
Illegal SpeI site found at 917
Illegal PstI site found at 140
Illegal PstI site found at 510
Illegal PstI site found at 3210 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal BglII site found at 852
Illegal BamHI site found at 158
Illegal BamHI site found at 2266
Illegal BamHI site found at 3130
Illegal BamHI site found at 3205 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal XbaI site found at 3113
Illegal SpeI site found at 917
Illegal PstI site found at 140
Illegal PstI site found at 510
Illegal PstI site found at 3210 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1399
Illegal EcoRI site found at 3216
Illegal XbaI site found at 3113
Illegal SpeI site found at 917
Illegal PstI site found at 140
Illegal PstI site found at 510
Illegal PstI site found at 3210
Illegal AgeI site found at 2084 - 1000COMPATIBLE WITH RFC[1000]