Difference between revisions of "Part:BBa K2936001"
(→‎Usage and Biology)
Line 17: Line 17:
  
 
===<h1>Usage and Biology</h1>===
 
===<h1>Usage and Biology</h1>===
−
 
+
<html>
 +
  <img src="https://static.igem.org/mediawiki/parts/d/d6/T--ZJUT-China--igem-part01fanying.png" alt=""width="600px">
 +
</html>
 +
<p>
 +
<br>
 +
Form 1 The ABS650 of reaction solution of bacterial culture and TMB
 +
<html>
 +
  <img src="https://static.igem.org/mediawiki/parts/d/d6/T--ZJUT-China--igem-part01fanying.png" alt=""width="600px">
 +
</html>
 +
<html>
 +
  <img src="https://static.igem.org/mediawiki/parts/4/47/T--ZJUT-China--igem-part01yanse.jpg" alt=""width="600px">
 +
</html>
 +
<br>
 +
Figure 1 The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right
 +
<br>
 +
From figure 1, the color of the third group is enough obvious compared to the background. And at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD650=0.5) to TMB is designed to be 1:10 .
 +
</p>
  
 
<!-- -->
 
<!-- -->

Revision as of 14:59, 16 October 2019


pBAD-LacZɑ

The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction.

The LacZ alpha gene is one of the structural genes present on the Lac Operon in E. coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene encoding β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) will be expressed.


Usage and Biology


Form 1 The ABS650 of reaction solution of bacterial culture and TMB
Figure 1 The ratio of ONPG is 1:2,1:4,1:8,1:10,1:15,1:20 from the left to right
From figure 1, the color of the third group is enough obvious compared to the background. And at the same time, in order to balance the principle of economy, the ratio of bacterial culture (OD650=0.5) to TMB is designed to be 1:10 .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 120
    Illegal BamHI site found at 182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]