Difference between revisions of "Part:BBa K3093000:Design"
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===Source=== | ===Source=== | ||
| − | + | ChbR was obtained from the genomic sequence of Escherichia coli str. K-12 substr.MG1655 | |
| − | Escherichia coli str. K-12 substr. MG1655 | + | |
===References=== | ===References=== | ||
Revision as of 14:17, 16 October 2019
chbR N238S Y30C
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
ChbR is encoded within the chb operon itself. In the presence of chitobiose, ChbR binds to two direct repeats located between two NagC binding sites and activates the transcription of the chb operon by overlapping the -35 box of chbBp. In the absence of chitobiose, ChbR acts as a repressor of the chb operon. Monoacetylchitobiose-6-P has been experimentally determined to be the inducer for ChbR.
Source
ChbR was obtained from the genomic sequence of Escherichia coli str. K-12 substr.MG1655