Difference between revisions of "Part:BBa K3241040"
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<partinfo>BBa_K3241040 short</partinfo> | <partinfo>BBa_K3241040 short</partinfo> | ||
− | + | This part is a new part, which consists of BBa_K3241000 BBa_K3241022 BBa_K3241112 BBa_K2800032 BBa_K2800013. It is used to construct the PHB biosynthetic pathway in Zymomonas mobilis . | |
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+ | ==Design Notes== | ||
− | + | <i>Zymomonas mobilis </i>is the model strain of ethanol production. It's mainly metabolize glucose, fructose and sucrose through ED(entner-doudoroff) pathway to produce ethanol. Here,we introduced phbA, phbB, and phbC genes from Ralstonia eutropha to modify its metabolic pathway.When Z.mobilis disassemble sugar molecules to produce pyruvate, One part of the Metabolic flux produces ethanol under the catalysis of pyruvate decarboxylase and ethanol dehydrogenase, and in another part pyruvate oxidation decarboxylation to acetyl CoA.Acetyl coenzyme A translates into PHB under the catalysis of β-ketothiolase, acetoacetyl-CoA Reductase and PHB polymerase. | |
+ | [[File:T--HUBU_WUHAN--HeterogenousPHB_biosynthesis_pathway.jpeg|300px|thumb|center|Fig.1 Heterogenous PHB biosynthesis pathway]] | ||
+ | [[File:T--HUBU_WUHAN--enzymes.jpg|300px|center|thumb|Fig.2 PHB synthesizes key enzymes]] | ||
+ | ===Part one: basic fermentation=== | ||
+ | We used the promter Ptet to explore the growth of recombinant strains under the induction of different concentrations of tetracycline.(the original can refer to BBa_K3241029) | ||
+ | [[File:T--HUBU WUHAN--Original design.png|300px|center|thumb| Fig.3 Original design.]] | ||
+ | [[File:T--HUBU WUHAN--Growth Curve aasd.jpg|500px|left|thumb| Fig.4 Growth Curve.]] | ||
+ | [[File:T--HUBU WUHAN--PHB Profuction.jpg|500px|right|thumb| Fig.5 PHB Production.]] | ||
+ | ==Sequence and Features== | ||
+ | <span class='h3bb'>Sequence and Features</span> | ||
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<partinfo>BBa_K3241040 SequenceAndFeatures</partinfo> | <partinfo>BBa_K3241040 SequenceAndFeatures</partinfo> | ||
Revision as of 22:01, 16 October 2019
Pgap-PhbC-PhbA-PhbB
This part is a new part, which consists of BBa_K3241000 BBa_K3241022 BBa_K3241112 BBa_K2800032 BBa_K2800013. It is used to construct the PHB biosynthetic pathway in Zymomonas mobilis .
Design Notes
Zymomonas mobilis is the model strain of ethanol production. It's mainly metabolize glucose, fructose and sucrose through ED(entner-doudoroff) pathway to produce ethanol. Here,we introduced phbA, phbB, and phbC genes from Ralstonia eutropha to modify its metabolic pathway.When Z.mobilis disassemble sugar molecules to produce pyruvate, One part of the Metabolic flux produces ethanol under the catalysis of pyruvate decarboxylase and ethanol dehydrogenase, and in another part pyruvate oxidation decarboxylation to acetyl CoA.Acetyl coenzyme A translates into PHB under the catalysis of β-ketothiolase, acetoacetyl-CoA Reductase and PHB polymerase.
Part one: basic fermentation
We used the promter Ptet to explore the growth of recombinant strains under the induction of different concentrations of tetracycline.(the original can refer to BBa_K3241029)
Sequence and Features
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 465
Illegal NheI site found at 1341
Illegal NheI site found at 3257
Illegal NotI site found at 2 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 495
Illegal NgoMIV site found at 2356
Illegal NgoMIV site found at 2441
Illegal NgoMIV site found at 2846
Illegal NgoMIV site found at 2975
Illegal AgeI site found at 600
Illegal AgeI site found at 3379
Illegal AgeI site found at 4051 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 421
Illegal BsaI site found at 1142
Illegal BsaI site found at 2272