Difference between revisions of "Part:BBa K2936012"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| + | ===<h1>Experiment</h1>=== | ||
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| + | Figure 1 (a) the pBAD gene of E. coli does include the binding sites of araC plasmid(b)the pBAD gene of E. coli does not include the binding sites of araC plasmid, LacZ alpha gene encodes the β-galactosidase, which could react with ONPG to generate orange color. | ||
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Revision as of 14:13, 16 October 2019
araBAD promoter
The promoter is a part of the arabinose operon whose name derives from the genes it regulates transcription of: araB, araA, and araD. In E. coli, the PBAD promoter is adjacent to the PC promoter, which transcribes the araC gene in the opposite direction. araC encodes the AraC protein, which regulates activity of both the PBAD and PC promoters.
Usage and Biology
Experiment
Figure 1 (a) the pBAD gene of E. coli does include the binding sites of araC plasmid(b)the pBAD gene of E. coli does not include the binding sites of araC plasmid, LacZ alpha gene encodes the β-galactosidase, which could react with ONPG to generate orange color.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 120
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]