Difference between revisions of "Part:BBa K3268000"
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1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus. | 1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus. | ||
<br> | <br> | ||
− | 2. Since the mOR103-15 coding sequence contains a BamHI cutting site, we | + | 2. Since the mOR103-15 coding sequence contains a BamHI cutting site, we had designed two PCR primers with HindIII and BglII sites respectively used for this amplification. |
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Revision as of 14:33, 16 October 2019
mOR103-15
This part codes for mouse olfactory receptor protein, which can bind with heptanal.
Our iGEM team had made a composite module that can highly express any inserted protein-coding genes (see BBa_K3268004).
We had used this composite module to highly express mOR103-15 (mouse olfactory receptor protein) in E. coli cells.
1. The mOR103-15 coding region was PCR amplified from the pI7-ISH plasmid (https://www.addgene.org/15527/) obtained from Addgene using high-fidelity enzyme KOD-Plus.
2. Since the mOR103-15 coding sequence contains a BamHI cutting site, we had designed two PCR primers with HindIII and BglII sites respectively used for this amplification.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 454
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 428
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 834