Difference between revisions of "Part:BBa K2936007"
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An alpha peptide chain encoded by a gene LacZ | An alpha peptide chain encoded by a gene LacZ | ||
| + | ===Description=== | ||
| + | Short description: Reporter gene.<br> | ||
| + | The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) ). | ||
| + | |||
| + | ===Source=== | ||
| + | <i> E. coli </I> | ||
| + | |||
| + | ===Design consideration=== | ||
| + | LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color. | ||
| + | |||
| + | ===Mechanism=== | ||
| + | https://static.igem.org/mediawiki/parts/d/d6/T--ZJUT-China--igem-part01fanying.png | ||
| + | |||
| + | ===Experiment=== | ||
===<h1>Usage and Biology</h1>=== | ===<h1>Usage and Biology</h1>=== | ||
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Revision as of 16:03, 16 October 2019
LacZ alpha
An alpha peptide chain encoded by a gene LacZ
Description
Short description: Reporter gene.
The LacZ alpha gene is one of the structural genes present on the Lac Operon in Escherichia coli bacteria . When transcribed by RNA polymerase , the LacZ alpha gene codes for β-galactosidase , an enzyme responsible for the hydrolysis of β-galactosides (e.g. 2-Nitrophenyl β-D-galactopyranoside (ONPG) ).
Source
E. coli
Design consideration
LacZ alpha gene encodes the β-galactosidase, which could react with 2-Nitrophenyl β-D-galactopyranoside (ONPG) to generate orange color.
Mechanism
