Difference between revisions of "Part:BBa K2984037"
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<partinfo>BBa_K2984037 short</partinfo> | <partinfo>BBa_K2984037 short</partinfo> | ||
| − | Level 1 backbone carrying the gene for the Phosphite oxidoreductase ( | + | <html> |
| − | + | This vector is a part of the <a href="https://2019.igem.org/Team:Humboldt_Berlin/Part_Collection">Chlamy-HUB-Collection</a>. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (<a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984013">PtxD</a>) which allows C.reinnhardtii to grow on phosphite. The trancriptional unit starts with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984008">PsaD promoter</a> and Ends with the <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984018">Rbcs2 terminator</a>. The construct contains a <a href="https://parts.igem.org/wiki/index.php?title=Part:BBa_K2984034">B2-B2</a> linker which connects the PsaD promoter to PtxD. | |
| + | <html> | ||
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<partinfo>BBa_K2984037 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2984037 SequenceAndFeatures</partinfo> | ||
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| + | ==Characterization== | ||
| + | <html> | ||
| + | <figure> | ||
| + | <img src="https://2019.igem.org/wiki/images/d/d2/T--Humboldt_Berlin--Konstruk_10.jpg" alt="colonies_total" width="500"> | ||
| + | <figcaption>Fig.1 - Image of a successful transformation in E.coli after ligation of the construct. The construct can then be isolated from E. coli to be transformed in C. reinhardtii</figcaption> | ||
| + | </figure> | ||
| + | </html> | ||
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Revision as of 10:11, 17 October 2019
L1c-Psad-PtxD-Rbcs2
This vector is a part of the Chlamy-HUB-Collection. Level 1 backbone carrying the gene for the Phosphite oxidoreductase (PtxD) which allows C.reinnhardtii to grow on phosphite. The trancriptional unit starts with the PsaD promoter and Ends with the Rbcs2 terminator. The construct contains a B2-B2 linker which connects the PsaD promoter to PtxD.
Sequence and Features
