Difference between revisions of "Part:BBa K2984036"

Revision as of 13:14, 16 October 2019

L1c-PsaD-PETase-YFP-RBCS2

This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a YFP mVenus marker (BBa_K2984024), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal PstI site found at 1915
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1244
Illegal PstI site found at 1915
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 4
Illegal XhoI site found at 968
23
INCOMPATIBLE WITH RFC[23]
Illegal PstI site found at 1915
25
INCOMPATIBLE WITH RFC[25]
Illegal PstI site found at 1915
Illegal NgoMIV site found at 2392
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K2984036 short</partinfo>
-This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a HA tag (BBa_K2984015), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
+This part is composed of the PsaD promoter (BBa_K2984008), a B2-B2 linker (BBa_K2984034), the PETase enzyme (), a YFP mVenus marker (BBa_K2984024), and the Rbcs2 terminator (BBa_K2984018). The part can be used to express the PETase enzyme in C. reinhardtii. The 3xHA tag can be used to purify and detect the PETase enzyme.
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