Difference between revisions of "Part:BBa K2940005"
Line 5: | Line 5: | ||
Coding sequence for Azoreductase AzoR with an N-terminal SpyCatcher. | Coding sequence for Azoreductase AzoR with an N-terminal SpyCatcher. | ||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
+ | This part is used for conjugation of AzoR on curli for enzyme immobilisation. The SpyCatcher is designed to be at the N-terminus of the AzoR enzyme. A 2x GGGGS flexible linker was introduced between AzoR and SpyCatcher to make sure the domains of two proteins were not interfering with each other. SpyCatcher sequence were acquired from iGEM registry (part: BBa_K1650037). And the flavin-free, oxygen insensitive azoreductase AzoR was acquired from NCBI (accession number: WP_014229193.1). | ||
<!-- --> | <!-- --> |
Revision as of 17:59, 21 October 2019
Coding Sequence of AzoR with an N-terminal SpyCatcher
Coding sequence for Azoreductase AzoR with an N-terminal SpyCatcher.
Usage and Biology
This part is used for conjugation of AzoR on curli for enzyme immobilisation. The SpyCatcher is designed to be at the N-terminus of the AzoR enzyme. A 2x GGGGS flexible linker was introduced between AzoR and SpyCatcher to make sure the domains of two proteins were not interfering with each other. SpyCatcher sequence were acquired from iGEM registry (part: BBa_K1650037). And the flavin-free, oxygen insensitive azoreductase AzoR was acquired from NCBI (accession number: WP_014229193.1).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 222
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]