Difference between revisions of "Part:BBa K3187018"

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Revision as of 11:33, 19 October 2019

PolyG Tag (GGGG) for Sortase-mediated Ligation

Profile

Name	PolyG-tag
Base pairs	12
Molecular weight	300 Da
Origin	Synthetic
Properties	second sequence for a Sortase A mediated reaction

Usage and Biology

The enzyme Sortase A is able to form a peptide bond between a C-terminal LPXTG motif (BBa_K3187019) and a N-terminal poly glycine motif ^{[1] [2] [3]} . The DNA sequence below is coding for four glycines that can be used as the second recognition motif in a Sortase reaction.

References

1. ↑ Tsukiji, S. and Nagamune, T. (2009) Sortase-Mediated Ligation: A Gift from Gram-Positive Bacteria to Protein Engineering [1]
2. ↑ Proft, T. (2010) Sortase-mediated protein ligation: an emerging biotechnology tool for protein modification and immobilisation [2]
3. ↑ Mao, H., Hart, S. A., Schink, A., and Pollok, B. A. (2004) Sortase-mediated protein ligation: a new method for protein engineering [3]

Sequence and Features