Difference between revisions of "Part:BBa K2908675"
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The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein. | The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein. | ||
| − | + | https://static.igem.org/mediawiki/parts/3/3a/T--CSU_CHINA--module2.png | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 21:37, 17 October 2019
s(ESR1)p-miR101spe-EGFP
The CSU_CHINA iGEM team 2019 designed a fusion protein consisting of miR101 sponge and EGFP. Therefore, we used our s(ESR1)p(BBa_K2908111) as a specific promoter.At the same time we used our miR101-sponge(BBa_K2908669) to bind different concentrations of miR101 and fuse it to 3' end so that can regulate the transactivation of EGFP which is a marker.In this way we can ensure the specificity and independence of this protein.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]