Difference between revisions of "Part:BBa K3110040:Design"

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===Source===
 
===Source===
  
−
sfGFP was obtained from the Biobrick. lldRO1-J23117-lldRO2+B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.
+
sfGFP along with Terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 and B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.
  
 
===References===
 
===References===

Revision as of 15:01, 16 October 2019


lldRO1-J23117-lldRO2 Strong RBS sfGFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 78
    Illegal NheI site found at 101
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 174


Design Notes

None.


Source

sfGFP along with Terminator was obtained from the Biobrick. lldRO1-J23117-lldRO2 and B0034 was obtained as gBlock from IDT and these were joined together using SOEing (Spliced Overlap Extension) PCR.

References